The trio trained separately from the group due to muscular problems on Thursday and were deemed unfit for selection by Diego Simeone a day later.
However, centre-back Jose Gimenez was cleared for duty after sustaining a thigh injury in the 1-1 draw at Villarreal on October 20.
Atletico will temporarily return to the top of LaLiga if they secure a victory at Butarque, though Barcelona could displace them later on Saturday when they travel to Rayo Vallecano.
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